Hold On For A Minute

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Elisa

The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are remov… more »

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 Watch: New Singing Lesson Videos Can Make Anyone A Great Singer

Nevermind if your legs are shaking
Nevermind all the words unspoken
All the promises that were broken
Soon enough they'll be gone
It ain't nothing but a good old handshake
Gotta leave them behind
Dark memories
All the bruises you showed nobody
Soon enough they'll be gone

You gotta keep it together
They're not doing you a favor
Now that you've tasted the flavor
It ain't sweet it's only bitter
You know you gotta try harder
You gotta keep it together
Watch it, 'cause soon they'll be gone

So hold on my friend
Stop crying
Just know that you can
Don't hold back again
Hold on for a minute
Hold on in a minute
The race will end and
It'll all come up to the surface
And the truth will be hot and burning
In your face like the sun

You gotta keep it together
They're not doing you a favor
Now that you've tasted the flavor
It ain't sweet it's only bitter
You know you gotta try harder
You gotta keep it together
Watch it, 'cause soon they'll be gone
So hold on my friend
Stop crying
Just know that you can
Don't hold back again
Hold on for a minute
You can

So hold on my friend
Hold on for a minute
Hold on for a minute
Hold on for a minute
Hold on for a minute
Hold on for a minute
Hold on (hold on)
You gotta hold on
Gotta hold on
Gotta hold on, hold on
Soon they'll be gone
So hold on my friend
Stop crying
Just know that you can
Don't you hold back again
Hold on for a minute
You can
So hold on my friend
Stop crying
Just know that you can
Don't you hold back again
Hold on
Hold on

 Watch: New Singing Lesson Videos Can Make Anyone A Great Singer