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The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are removed. In the final step, a substance containing the enzyme's substrate is added. If there was binding, the subsequent reaction produces a detectable signal, most commonly a color change. Performing an ELISA involves at least one antibody with specificity for a particular antigen. The sample with an unknown amount of antigen is immobilized on a solid support (usually a polystyrene microtiter plate) either non-specifically (via adsorption to the surface) or specifically (via capture by another antibody specific to the same antigen, in a "sandwich" ELISA). After the antigen is immobilized, the detection antibody is added, forming a complex with the antigen. The detection antibody can be covalently linked to an enzyme or can itself be detected by a secondary antibody that is linked to an enzyme through bioconjugation. Between each step, the plate is typically washed with a mild detergent solution to remove any proteins or antibodies that are non-specifically bound. After the final wash step, the plate is developed by adding an enzymatic substrate to produce a visible signal, which indicates the quantity of antigen in the sample. Of note, ELISA can perform other forms of ligand binding assays instead of strictly "immuno" assays, though the name carried the original "immuno" because of the common use and history of development of this method. The technique essentially requires any ligating reagent that can be immobilized on the solid phase along with a detection reagent that will bind specifically and use an enzyme to generate a signal that can be properly quantified. In between the washes, only the ligand and its specific binding counterparts remain specifically bound or "immunosorbed" by antigen-antibody interactions to the solid phase, while the nonspecific or unbound components are washed away. Unlike other spectrophotometric wet lab assay formats where the same reaction well (e.g., a cuvette) can be reused after washing, the ELISA plates have the reaction products immunosorbed on the solid phase, which is part of the plate, and so are not easily reusable.

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Albums by ElisaSort:By AlbumA - Z


Birds [2019]

SongDuration
Birds3:39

Secret Diaries [2019]

SongDuration
A Parallel World3:04

Vietato Morire [2017]

SongDuration
Piccola anima3:41

Love 2016 [2016]

SongDuration
Una poesia anche per te5:15

NRJ Dance Hits 2016 [2016]

SongDuration
No Hero4:13

NRJ Summer Hits Only 2016 [2016]

SongDuration
No Hero4:13

Django Unchained [Clean] [2012]

SongDuration
Ancora Qui5:08

Love Is Requited [2011]

SongDuration
Love Is Requited3:37

Casa 69 [2010]

SongDuration
Basta Così5:59

Special Edition [2010]

SongDuration
Basta Così5:59

Gift: The Remixes [2007]

SongDuration
Gift8:10

Basi Mus X Teenag [2005]

SongDuration
Waves 

Swan [2005]

SongDuration
Swan4:14
Rainbow4:10

Time [2002]

SongDuration
Time3:56

Blu

SongDuration
Blu2:45

Unknown Album

SongDuration
Se Piovesse Il Tuo Nome3:22
Tua Per Sempre3:27
L'Estate È Già Fuori3:20
Vivere Tutte Le Vite3:47
Thirst (For You Only)3:21
Soul4:14
Con Te Mi Sento Così3:03
Destinazione Paradiso3:49
No Hero 
Birds [Remix]3:39
A Parallel World3:04
Medo De Sentir2:54
NEON - Le Ali3:27
Andrà Tutto Bene3:27
Three Little Birds3:03
YO NO TENGO NADA3:06
Hallelujah7:26
Sentir Sin Embargo 
The Big Dipper4:02
Together4:22
Bitter Words3:18
Bruciare Per Te 
Life Goes On 
Coincidences4:05
Broken4:20
A Modo Tuo 
Poesia Anche Per Te5:14
Someone To Love3:33
Dot In the Universe4:37
The Marriage4:21
Forgiveness4:26
Vortexes3:36
One 
Poems By God3:51
Time3:56
Nostalgia4:54
Upside Down4:10
Ti Vorrei Sollevare4:27
A Little Over Zero5:01
Hold On For A Minute3:08
Basta Così5:58
L'anima vola4:03
Seven Times 
Qualcosa Che Non C'è 
Dancing [Edit]3:36
Lisert6:34
Fever4:19
Rain Over My Head3:58
A Feast for Me5:12
I Know4:11
Wild Horses5:55
Come Speak To Me4:24
City Lights3:53
LOVE AS A KINDA WAR 
Blu Part II - con Rkomi3:35
Bad Habits3:44
Blu2:45
Mad World5:15
Volente o Nolente3:38
We Will Be Strangers3:38
Electricity4:10
Un filo di seta negli abissi3:37
Pagina Bianca 
Teach Me Again [Elisa Version]4:42
Rainbow4:30
Anche Tu, Anche Se (Non Trovi Le Parole) 
Sleeping In Your Hand [Mark Saunders Remix] 
Maledetto labirinto 
Asile's World3:50
Stay4:10
Fresh Air3:11
Ti Dirò Di Sì 
Inside a Flower 
Non fa niente ormai4:58
Eppure Sentire (Un Senso di Te)4:13
Femme Fatale4:40
This Knot3:22
Love Me Forever3:16
Heaven Out of Hell 
Love Is Requited3:37
Gli Ostacoli del Cuore4:03
19794:22
Pour Que L'amour Me Quitte3:15
Happiness is Home4:55
Gift4:22
Labyrinth 
Bridge Over Troubled Water 
Redemption Song5:23
(Sittin On) The Dock of the Bay2:48
Almeno Tu Nell' Universo4:09
Rock Your Soul5:05
Rubini3:48
Ecco che3:54
Pagina Bianca [Radio Version]3:51
C'eravamo Tanto Amati4:15
L' Abitudine Di Sorridere5:02
Maledetto labirinto [Radio Edit]4:09
Da Sola / In the Night3:10
Birds3:39
Shout1:33
Mare Mare3:10
Olvidar3:43
REALISM4:48
そばにいるよ4:13
La Mariposa y el Doctor4:36
Sin Querer (feat. Nicolas Chandia)3:21
Hombre Ciego3:25
Lasciati4:15
Realize3:01
Cosas Que Sé3:48
ex:tella4:35
Mi Mascara y Persona3:28
Piedad3:14
Auschwitz5:10
Luce (Tramonti a Nord Est)4:42
Seta3:33
Escapé4:10
Senza Sogni2:56
LA VIE 
Simplemente4:01
Intenciones3:21
Casi3:05
Physical3:29
Sigues Siendo Así3:44
Tengo Incesantes Ganas3:38
Una Poesia Anche Per Te (Life Goes On) 
A Tempo Perso3:47
So Much of Me (Gli Ostacoli Del Cuore)4:19
Teach Me Again 
Drifting4:07
Endless Night2:18
Chi Lo Sa3:29
Palla Al Centro3:47
Hablame 
Just as One3:48
Quando Arriva La Notte4:18
Litoranea3:33
No One Compares (feat. Outlaw jacc)3:38
Mira Lo Que Haces2:15
Bloody Mary4:11
Come Te Nessuno Mai4:46
Non Me Ne Pento3:22
Quello Che Manca3:30
O Forse Sei Tu3:53
Show's Rollin'3:48
Luglio3:37
One Step Away (Eppure Sentire)3:42
Estrellita2:50
D10S3:49
Amigo Bronco3:47
Ordinary Day3:50
Come Sei Veramente3:56
Like I Want You2:41
Pugni Sotto La Cintura3:55
Lento3:09
Quero Bailar2:28
Fire4:22
Written In Your Eyes6:54
Wonder Wind4:03
L'Amore È Nell'Aria Stasera3:02
Let Me3:51
ミレナリオ4:57
Tienimi Con Te4:34
A Prayer4:35
Diari Aperti3:48
Monet3:18
Stranger3:54
Hope3:47
Like Children (feat. Liliia Kysil)3:46
Let It Go To Waste On Me3:59
Domino3:34
I Feel It In The Earth3:21
A muso duro4:52
Diamanti3:30
Set The Tone3:37
Così Non Va3:51
Creature4:10
Lullaby 
A Modo Tuo [Radio Edit]4:07
On Me3:32
Tilt3:24
Silent Song3:55
Expectativas4:01
Se Não Me Amas 
Canzoni per gli altri3:50
What I Like (feat. Broskrr Bleuw)3:06
Secret Labyrinth4:35
Luce (Tramonti A Nord Est) [Recordings at Abbey Road Studios]4:20
Dancing [Recordings at Abbey Road Studios]5:45
Labyrinth [Recordings at Abbey Road Studios]5:06
Silent Night [Recordings at Abbey Road Studios]4:26
Together [Recordings at Abbey Road Studios]4:17
Eppure Sentire (Un Senso Di Te) [Recordings at Abbey Road Studios]3:42
Una Poesia Anche Per Te (Life Goes On) [Recordings at Abbey Road Studios]5:27
No Hero [Recordings at Abbey Road Studios]4:18
Se Piovesse Il Tuo Nome [Recordings at Abbey Road Studios]3:25
Ancora Qui 
Overture / Un Filo Di Seta Negli Abissi [Recordings at Abbey Road Studios]4:57
Ancora Qui [Recordings at Abbey Road Studios]4:55
Buon Natale Anche A Te [Recordings at Abbey Road Studios]3:35
L'Anima Vola [Recordings at Abbey Road Studios]4:09
Quando Nevica3:28
A Modo Tuo [Recordings at Abbey Road Studios]5:19
O Forse Sei Tu [Recordings at Abbey Road Studios]3:52
Ogni Istante3:46
Qualcosa Che Non C'è [Recordings at Abbey Road Studios]4:35
Quelli Che Restano4:25

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